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16 60 sephacryl tm s 300  (Cytiva Europe)


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    Structured Review

    Cytiva Europe 16 60 sephacryl tm s 300
    16 60 Sephacryl Tm S 300, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiprep+16+60+sephacryl+s+300/HiPrep+16%2F60+Sephacryl+S-300+HR+column/10__1016_slash_j__biteb__2026__103151-63-0-5
    Average 94 stars, based on 81 article reviews
    16 60 sephacryl tm s 300 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Digital seed amplification assay for TDP-43 aggregate quantification in CSF.
    Article Snippet: The supernatant was diluted 1:1 in binding buffer and run through a His-trap Sepharose column (Cytiva, #17524802), and the protein was eluted via a linear imidazole gradient from 5 mM to 500 mM. .. The target protein was further purified using size exclusion chromatography (SEC) with an AKTA PureTM protein purification system on a HiPrep 16/60 Sephacryl S-300 HR (Cytiva, #17116701) column with TBS (10 mM Tris, 500 mM NaCl), 4 M urea, pH 7.5 as the mobile phase. ..

    Article Title: The kinesin Kip2 promotes microtubule growth using a bipartite polymerase module to deliver tubulin to microtubule plus ends.
    Article Snippet: .. The proteins were further purified by size exclusion chromatography using the Akta Start system with a HiPrep 16/60 Sephacryl S-300 HR column (Cytiva). .. The truncated version of KIF5B (Kinesin-1) [KIF5B(1–560)] from the organism Mus musculus was obtained by PCR amplification of the region from the plasmid pKin1B [pKin1B was a gift from Anthony Brown42 (Addgene plasmid #31604; http://n2t.net/addgene: 31604; RRID:Addgene_31604)].

    Article Title: Single-molecule imaging reveals DNA shape read-out by the INO80 chromatin remodeler
    Article Snippet: .. Oligos containing biotin (5'-P-AGGTCGCCGCCC-bio-3') or digoxigenin (5'-P-GGGCGGCGACCT-dig-3') were hybridized to the cos sites of the lambda DNA, ligated overnight at room temperature using T4 ligase (NEB), and purified using a HiPrep 16/60 Sephacryl S-300 HR column (Cytiva) in TE150 buffer. .. For the assembly of nucleosomes, λ -DNA constructs were concentrated using Amicon Ultra-15 filters (30 kDa MWCO, Merck).

    Article Title: Tunable supramolecular polymerization from protein charge heterogeneity and architecture
    Article Snippet: .. His-Bem1-Flag and His-Bem11-464-Flag were further purified by size exclusion chromatography on a HiPrep 16/60 Sephacryl S-300 HR (Cytiva) column using SEC buffer. ..

    Article Title: Dynamic Lysine Acetylation Disrupts Isocitrate Lyase Function and Enables Metabolic Optimisation.
    Article Snippet: The eluted proteins were buffer- exchanged into conservation buffer (50 mM potassium phosphate, 100 mM NaCl, pH 8) using a HiPrep 26/10 desalting column (GE Healthcare) at 9 mL min−1. .. Purified ICL and its variants were concentrated and analysed by size- exclusion chromatography on a HiPrep 16/60 Sephacryl S- 300 HR column (GE Healthcare) at 1 mL min−1 using a 15– 600 kDa calibration kit (Sigma). ..

    Size-exclusion Chromatography:

    Article Title: Digital seed amplification assay for TDP-43 aggregate quantification in CSF.
    Article Snippet: The supernatant was diluted 1:1 in binding buffer and run through a His-trap Sepharose column (Cytiva, #17524802), and the protein was eluted via a linear imidazole gradient from 5 mM to 500 mM. .. The target protein was further purified using size exclusion chromatography (SEC) with an AKTA PureTM protein purification system on a HiPrep 16/60 Sephacryl S-300 HR (Cytiva, #17116701) column with TBS (10 mM Tris, 500 mM NaCl), 4 M urea, pH 7.5 as the mobile phase. ..

    Article Title: Microfluidic measurement of the size and shape of lipid-anchored proteins.
    Article Snippet: The surface of a cell is crowded with membrane proteins.. The size, shape, density, and mobility of extracellular surface proteins mediate cell surface accessibility to external molecules, viral particles, and other cells.. However, predicting these qualities is not always straightforward, even when protein structures are known.

    Article Title: The kinesin Kip2 promotes microtubule growth using a bipartite polymerase module to deliver tubulin to microtubule plus ends.
    Article Snippet: .. The proteins were further purified by size exclusion chromatography using the Akta Start system with a HiPrep 16/60 Sephacryl S-300 HR column (Cytiva). .. The truncated version of KIF5B (Kinesin-1) [KIF5B(1–560)] from the organism Mus musculus was obtained by PCR amplification of the region from the plasmid pKin1B [pKin1B was a gift from Anthony Brown42 (Addgene plasmid #31604; http://n2t.net/addgene: 31604; RRID:Addgene_31604)].

    Article Title: Tunable supramolecular polymerization from protein charge heterogeneity and architecture
    Article Snippet: .. His-Bem1-Flag and His-Bem11-464-Flag were further purified by size exclusion chromatography on a HiPrep 16/60 Sephacryl S-300 HR (Cytiva) column using SEC buffer. ..

    Article Title: Dynamic Lysine Acetylation Disrupts Isocitrate Lyase Function and Enables Metabolic Optimisation.
    Article Snippet: The eluted proteins were buffer- exchanged into conservation buffer (50 mM potassium phosphate, 100 mM NaCl, pH 8) using a HiPrep 26/10 desalting column (GE Healthcare) at 9 mL min−1. .. Purified ICL and its variants were concentrated and analysed by size- exclusion chromatography on a HiPrep 16/60 Sephacryl S- 300 HR column (GE Healthcare) at 1 mL min−1 using a 15– 600 kDa calibration kit (Sigma). ..

    Protein Purification:

    Article Title: Digital seed amplification assay for TDP-43 aggregate quantification in CSF.
    Article Snippet: The supernatant was diluted 1:1 in binding buffer and run through a His-trap Sepharose column (Cytiva, #17524802), and the protein was eluted via a linear imidazole gradient from 5 mM to 500 mM. .. The target protein was further purified using size exclusion chromatography (SEC) with an AKTA PureTM protein purification system on a HiPrep 16/60 Sephacryl S-300 HR (Cytiva, #17116701) column with TBS (10 mM Tris, 500 mM NaCl), 4 M urea, pH 7.5 as the mobile phase. ..

    Buffer Exchange:

    Article Title: Microfluidic measurement of the size and shape of lipid-anchored proteins.
    Article Snippet: The surface of a cell is crowded with membrane proteins.. The size, shape, density, and mobility of extracellular surface proteins mediate cell surface accessibility to external molecules, viral particles, and other cells.. However, predicting these qualities is not always straightforward, even when protein structures are known.

    DNA Ligation:

    Article Title: Role of Aspartate 86 in the Catalytic Mechanism of Escherichia coli Glutamate Decarboxylase
    Article Snippet: The GeneTailor Site-Directed Mutagenesis system (Invitrogen), d , l -Norvaline (Alfa Aesar), Taq DNA polymerase (used for colony screening of mutant clones), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein markers were from Thermo Fisher Scientific. .. The DNA ligation system, prepacked HiPrep DEAE FF 16/10 (20 mL), and HiPrep 16/60 Sephacryl S-300 HR were from GE Healthcare. .. Kits for plasmid DNA purification and DNA extraction from agarose gel were from Macherey-Nagel.

    Lambda DNA Preparation:

    Article Title: Single-molecule imaging reveals DNA shape read-out by the INO80 chromatin remodeler
    Article Snippet: .. Oligos containing biotin (5'-P-AGGTCGCCGCCC-bio-3') or digoxigenin (5'-P-GGGCGGCGACCT-dig-3') were hybridized to the cos sites of the lambda DNA, ligated overnight at room temperature using T4 ligase (NEB), and purified using a HiPrep 16/60 Sephacryl S-300 HR column (Cytiva) in TE150 buffer. .. For the assembly of nucleosomes, λ -DNA constructs were concentrated using Amicon Ultra-15 filters (30 kDa MWCO, Merck).



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